R5773
Anti-Raf-1 (631-648) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 74 kDa
species reactivity
human, rat
technique(s)
immunoprecipitation (IP): 5 μg using 10 μg lysate of human epidermal carcinoma A431 cells
microarray: suitable
western blot: 1:8,000 using a human epidermal carcinoma A431 cell line lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... RAF1(5894)
rat ... Raf1(24703)
General description
Anti-Raf-1 is developed in rabbits using a synthetic peptide K-TEDINACTLTTSPRLPVF corresponding to the C-terminal of Raf-1 of human origin, conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Raf-1 (c-Raf-1), a cytoplasmic serine/threonine protein kinase (73-76kD), belongs to a small family of protein kinases. Raf-1 is the prototype member of this family that include Raf-A and Raf-B and is the cellular homolog of the viral oncogene v-Raf. Raf1 is widely expressed in many tissues, whereas Raf-A and Raf-B are expressed mainly in the urogenital and brain tissues, respectively.
Immunogen
synthetic peptide corresponding to amino acids 631-648 of human Raf-1.
Application
Anti-Raf-1 (631-648) antibody produced in rabbit has been used in:
- immunolabeling assays
- western blotting
- immunoprecipitation
Biochem/physiol Actions
Raf-1 (c-Raf-1) functions in the control of cell growth, differentiation and its oncogenic form can initiate malignant transformation of cells. Raf-1 has a critical role in the Ras/MAP kinase signaling pathway, integrating mitogenic signals mammalian cells from many growth factors, cytokines and oncogenes including epidermal growth factor (EGF), platelet-derived growth factor (PDGF), insulin, interleukin 2 (IL-2), IL-3, colony-stimulating factor 1 (CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF). Raf-1 regulates the MAP kinase pathway by phosphorylating and activating the downstream MAP kinase kinase, MEK. c-Raf kinase is the first identified downstream effector kinase of Ras and is the activator of MEK which in turn activates ERK.
The sequence of the immunizing peptide is identical in rat Raf-1 and v-Raf from the mouse sarcoma virus. Anti-Raf-1 specifically recognises Raf-1 (74 kDa). The antibody may also detect Raf-1 degradation products at 40-60 kDa.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Striatal GDNF administration increases tyrosine hydroxylase phosphorylation in the rat striatum and substantia nigra
Salvatore MF, et al.
Journal of Neurochemistry, 90(1), 245-254 (2004)
Takuya Hasegawa et al.
FEBS open bio, 7(8), 1154-1165 (2017-08-07)
We previously reported that microRNA-205 (miR-205) is downregulated by overexpression of the receptor tyrosine kinase ErbB2 and that ectopic transfection of miR-205 precursor decreases ErbB2 tumorigenicity in soft agar. In this study, we further analyzed the regulatory mechanisms linking ErbB2
MEK-1 Activates C-Raf Through a Ras-Independent Mechanism
Leicht DT, et al.
Biochimica et Biophysica Acta, 1833(5), 976-976 (2013)
Trihydrophobin 1 is a new negative regulator of A-Raf kinase
Liu W, et al.
The Journal of biological chemistry, 279(11), 10167-10175 (2004)
Raf Kinases: Function, Regulation and Role in Human Cancer
Leicht DT, et al.
Biochimica et Biophysica Acta, 1773(8), 1196-1196 (2007)
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