R6004
Ava II from Anabaena variabilis
buffered aqueous glycerol solution
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
5,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
AvaII is a restriction enxyme used in molecular biology applications to cleave the DNA recognition sequence 5′-G/G(A,T)CC-3′, generating fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-G/G(A,T)CC-3′
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA, 50% glycerol (v/v) at 4°C
Other Notes
Comment: Activity of Ava II blocked by overlapping dcm methylation
Supplied with 10x Restriction Endonuclease Buffer SA (B7531).
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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M Nannipieri et al.
Journal of the American Society of Nephrology : JASN, 10(7), 1530-1541 (1999-07-15)
Approximately 30% of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and
K Murray et al.
The Biochemical journal, 159(2), 317-322 (1976-11-01)
Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
D S Jansson et al.
British poultry science, 53(6), 790-799 (2013-02-13)
1. Vancomycin-resistant Enterococcus faecium (VRE(fm)) has recently spread among Swedish broiler farms. The objectives were to investigate VRE(fm) persistence within barns between flocks, and to determine whether day-old chicks, feed or forklift trucks used for loading crates could be identified
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