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R6379

Xho I from Xanthomonas holcicola

Name: <I>Xho</I> I from <I>Xanthomonas holcicola</I>
Brand: SIGMA

Restriction Enzyme

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About This Item

CAS Number:

81295-43-4

UNSPSC Code:

12352204

MDL number:

MFCD00166586

EC Number:

3.1.21.4 (BRENDA, IUBMB)

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Properties

grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Description

Application

XhoI is a resteriction endonuclease used for molecular biology methods to cut DNA molecules at the recognition sequence 5′-C/TCGAG-3′ to generate fragments with 5′-cohesive ends.

Biochem/physiol Actions

Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Polydocanol (v/v) at 4 °C

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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