R7135
Sph I from Streptomyces phaeochromogenes
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-GCATG/C-3′
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl , pH 8.2, 1 mM EDTA, 350 mM NaCl, 7 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Characterization of a site-specific restriction endonuclease Sph l from Streptomyces phaeochromogenes.
Fuchs, L.Y., et al.
Gene, 10, 391-391 (1980)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Diana Schenkwein et al.
Nucleic acids research, 41(5), e61-e61 (2013-01-01)
Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci.
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service