R7763
Cla I from Caryophanon latum L
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
ClaI is a restriction enzyme that cleaves the DNA recognition sequence 5′-AT/CGAT-3′, generating DNA fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-AT/CGAT-3′
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Cla I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Isoschizomer: BspD I
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Maria Maguire et al.
Cancer research, 68(9), 3232-3242 (2008-05-03)
MDM2 is a ubiquitin ligase that is best known for its essential function in the negative regulation of p53. In addition, MDM2 expression is associated with tumor progression in a number of common cancers, and in some cases, this has
H Mayer et al.
Nucleic acids research, 9(19), 4833-4845 (1981-10-10)
From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
D S Jansson et al.
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