R7763
Cla I from Caryophanon latum L
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Application
ClaI is a restriction enzyme that cleaves the DNA recognition sequence 5′-AT/CGAT-3′, generating DNA fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-AT/CGAT-3′
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Cla I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Isoschizomer: BspD I
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
H Mayer et al.
Nucleic acids research, 9(19), 4833-4845 (1981-10-10)
From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates
Maria Maguire et al.
Cancer research, 68(9), 3232-3242 (2008-05-03)
MDM2 is a ubiquitin ligase that is best known for its essential function in the negative regulation of p53. In addition, MDM2 expression is associated with tumor progression in a number of common cancers, and in some cases, this has
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service