R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
Synonym(s):
Transfer RNA, tRNA
grade
Molecular Biology
Quality Level
type
Type V
form
lyophilized powder
specific activity
15-19 units/mg solid
storage condition
desiccated
solubility
H2O: ~2 mg/mL, clear to hazy
storage temp.
2-8°C
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General description
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Preparation Note
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Other Notes
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
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