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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
9014-25-9
MDL number:
MFCD00132200
NACRES:
NA.55

grade

for molecular biology

Quality Level

300

type

Type V

form

lyophilized powder

specific activity

15-19 units/mg solid

storage condition

desiccated

solubility

H2O: ~2 mg/mL, clear to hazy

storage temp.

2-8°C

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General description

Wheat germ tRNA contains many different specific tRNA molecules.

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Unit Definition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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