R8000
D-Ribulose 1,5-Diphosphate Carboxylase from spinach
partially purified powder, 0.01-0.1 unit/mg solid
Synonym(s):
3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
0.01-0.1 unit/mg solid
Biological source:
spinach
biological source
spinach
Quality Level
form
partially purified powder
specific activity
0.01-0.1 unit/mg solid
mol wt
557 kDa
storage temp.
−20°C
General description
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
Application
D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
- as a test protein in pepsin digestion studies
- as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
- in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid
Biochem/physiol Actions
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.
Other Notes
One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Related Content
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Regulatory toxicology and pharmacology : RTP, 39(2), 87-98 (2004-03-26)
Rationale. Evaluation of the potential allergenicity of proteins derived from genetically modified foods has involved a weight of evidence approach that incorporates an evaluation of protein digestibility in pepsin. Currently, there is no standardized protocol to assess the digestibility of
Martin A J Parry et al.
Journal of experimental botany, 64(3), 717-730 (2012-11-20)
Rubisco (ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase) enables net carbon fixation through the carboxylation of RuBP. However, some characteristics of Rubisco make it surprisingly inefficient and compromise photosynthetic productivity. For example, Rubisco catalyses a wasteful reaction with oxygen that leads to the release
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Although ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was discovered nearly 60 years ago, the associated chemical mechanism of the reaction is still incompletely understood. The catalytic cycle consists of four major steps: ribulose-1,5-bisphosphate binding, enolization, CO₂ or O₂ addition and hydration, and cleavage
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R8000-5UN | 04061836807375 |
| R8000-1UN | 04061836807368 |