R8154
Monoclonal Anti-RAP1 antibody produced in mouse
~2 mg/mL, clone 4C8/1, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-TERF2-Interacting Protein, Anti-TERF2IP
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4C8/1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~47 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TERF2IP(54386)
General description
Monoclonal Anti-RAP1 (mouse IgG2b isotype) is derived from the hybridoma 4C8/1 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with human RAP1. Ras-related protein 1 (RAP1) protein contains 399 amino acids with an acidic C-terminus featuring a 33 amino acid coiled-coil region and a bipartite nuclear localization signal. It is expressed ubiquitously, is located at telomeres and affects telomer length.
Rap1 (repressor/activator protein) is a constituent of shelterin. It consists of an N-terminal BRCT motif, a C-terminal protein interaction domain, and one or two central Myb domains. It is also known as Terf2ip (TERF2 interacting protein). It is located on chromosome 16q23.1.
Immunogen
human RAP1.
Application
Monoclonal Anti-RAP1 antibody produced in mouse may be used in enzyme linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Rap1 (repressor/activator protein) helps to guard telomeres, assemble telomerase, and controls telomere length. At telomeres, Rap1 also participates in the suppression of non-homologous end joining (NHEJ). It involves in genomewide transcriptional modulation. Knockdown of Rap1 induces apoptosis in hepatocellular carcinoma cell line HepG2.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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TALEN gene knockouts reveal no requirement for the conserved human shelterin protein Rap1 in telomere protection and length regulation
Kabir S, et al.
Cell Reports, 9(4), 1273-1280 (2014)
Genetic variants in eleven telomere-associated genes and the risk of incident cardio/cerebrovascular disease: The Women's Genome Health Study
Zee RY, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 412(1-2), 199-202 (2011)
Decoding telomere protein Rap1: Its telomeric and nontelomeric functions and potential implications in diabetic cardiomyopathy
Cai Y, et al.
Cell Cycle, 16(19), 1765-1765 (2017)
Mammalian RAP1 controls telomere function and gene expression through binding to telomeric and extra-telomeric sites
Martinez P, et al.
Nature Cell Biology, 12(8), 768?780-768?780 (2010)
Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2
Zha Y, et al.
Oncology Reports, 31(4), 1691-1698 (2014)
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