R8257
Mlu I from Micrococcus luteus (lysodeikticus)
buffered aqueous glycerol solution
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Disclaimer
Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
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New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI).
H Sugisaki et al.
Gene, 16(1-3), 73-78 (1981-12-01)
Two new restriction endonucleases have been isolated from Flavobacterium okeanokoites IFO12536 and Micrococcus luteus IFO12992 and named FokI and MluI, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Lilia Gabsalilow et al.
Nucleic acids research, 41(7), e83-e83 (2013-02-15)
Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions
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