R8501
Ribonucleic acid-core from baker′s yeast (S. cerevisiae)
Type XI-C
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About This Item
type
Type XI-C
storage temp.
−20°C
Application
Ribonucleic acid (RNA) from baker′s yeast S. cerevisiae may be used as a substrate for studying ribonuclease activities of enzymes such as RNAase(s).
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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T V Iamkovaia et al.
Prikladnaia biokhimiia i mikrobiologiia, 42(1), 93-97 (2006-03-09)
A simple method of production of total RNA from baker's yeast was developed. Total RNA was isolated from yeast (Saccharomyces cerevisiae) biomass using lysis with sodium dodecyl sulfate at 100 degrees C for 40-60 min and subsequent precipitation of the
Xiangli Wu et al.
The Journal of general and applied microbiology, 56(3), 231-239 (2010-07-22)
A 15-kDa RNase was purified from Pleurotus djamor using ion exchange chromatography and gel filtration. Its N-terminal amino acid sequence was different from previously reported RNase sequences of mushrooms belonging to the genus of Pleurotus and other genera. The RNase
RNA immunoprecipitation for determining RNA-protein associations in vivo.
Gilbert C, Svejstrup JQ.
Current Protocols in Molecular Biology, Unit 27-Unit 27 (2006)
Evandro Fei Fang et al.
FEBS letters, 584(18), 4089-4096 (2010-09-08)
A pumpkin 2S albumin with ribonuclease (RNase) activity was purified from pumpkin seeds (Cucurbita sp.) by liquid chromatographic techniques. It manifested potent RNase activity toward baker's yeast RNA and calf liver RNA, and some polyhomoribonucleotides, including poly(A), poly(U) and poly(C)
The preparation and characterization of ribonucleic acids from yeast.
A M CRESTFIELD et al.
The Journal of biological chemistry, 216(1), 185-193 (1955-09-01)
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