R9376
AMV-Reverse Transcriptase from avian myeloblastosis virus
Synonym(s):
RNA-directed DNA polymerase, Revertase
form
solution
concentration
10,000-100,000 units/mL
shipped in
dry ice
storage temp.
−70°C
Application
AMV reverse transcriptase synthesizes DNA complementary (cDNA) to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.
Other Notes
One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Regulatory Information
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Xiu-Juan Jiao et al.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 26(6), 453-455 (2013-05-01)
To characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. Serum samples of 229 CHB patients with NA treatment were obtained. Full-length HBV RT sequences were amplified, sequenced and analyzed, on
Ausubel, F., et al.
Short Protocols in Molecular Biology, 14-14 (1995)
TERT hypermethylation: biomarker in paediatric brain tumours.
Scott J Diede
The Lancet. Oncology, 14(6), 447-448 (2013-04-20)
Jiansen Jiang et al.
Nature, 496(7444), 187-192 (2013-04-05)
Telomerase adds telomeric repeats to chromosome ends using an internal RNA template and a specialized telomerase reverse transcriptase (TERT), thereby maintaining genome integrity. Little is known about the physical relationships among protein and RNA subunits within a biologically functional holoenzyme.
Yang Li et al.
RNA (New York, N.Y.), 19(6), 852-860 (2013-04-16)
Telomerase is a ribonucleoprotein (RNP) enzyme essential for telomere maintenance and chromosome stability. While the catalytic telomerase reverse transcriptase (TERT) protein is well conserved across eukaryotes, telomerase RNA (TR) is extensively divergent in size, sequence, and structure. This diversity prohibits
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