R9507
Taq I from Thermus aquaticus
recombinant, expressed in E. coli (Strain that carries a Taq I overproducing plasmid.), Restriction Enzyme
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About This Item
recombinant
expressed in E. coli (Strain that carries a Taq I overproducing plasmid.)
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
TaqI is a restriction endonuclease used in molecular biology applications to cleave DNA moledcules at the recognition site 5′-T/CGA-3′, generating fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Taq I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). Taq I is inefficient in digesting single stranded DNA . Overlapping dam methylation will block cleavage by Taq I. Activity of the enzyme is enhanced by BSA and higher temperatures, >37 °C. Optimal temperature is 65 °C.
Supplied with 10x Restriction Enzyme Buffer SB (B8781).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Katherine Paola Luna-Marín et al.
Parasitology research, 105(2), 519-528 (2009-04-07)
Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on
E Reizenstein et al.
Journal of clinical microbiology, 34(4), 810-815 (1996-04-01)
A nested PCR, using a 239-bp sequence in the pertussis toxin promoter region, was developed and evaluated. The assay differentiates Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by restriction enzyme analysis of the amplified fragments. The diagnostic performance of the
S Sato et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 542-546 (1977-02-01)
A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and bacteriophage psiX174 RF DNA at 10 sites. The enzyme