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RAB0005

Sigma-Aldrich

Human ADIPOQ / Adiponectin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):
ACDC, ADPN, ACRP30, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein, Adipose most abundant gene transcript 1 protein
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 24.69-18000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ADIPOQ(9370)

General description

The Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Adiponectin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Adiponectin

Application

Human ADIPOQ / Adiponectin ELISA Kit has been used in the quantification of leptin and adiponectin from serum and plasma samples.
For research use only. Not for use in diagnostic procedures.
Human ADIPOQ / Adiponectin ELISA Kit has been used for the measurement of adiponectin in human plasma.

Biochem/physiol Actions

Adiponectin (ADIPOQ) is an adipocyte specific secreted protein that circulates in the plasma. The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain. It has anti-atherosclerotic and anti-inflammatory properties. ADIPOQ has a role in physiological processes such as energy homeostasis and obesity. It exhibits anti-diabetic action by interacting with adiponectin receptors, AdipoR1 and AdipoR2. Low levels of adiponectin are also linked with hypertension and dyslipidemia. Mutation in the gene encoding it is linked with risk for endometrial cancer.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Min Wu et al.
Frontiers in pharmacology, 11, 223-223 (2020-04-02)
Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota. Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated.
Heli Viljakainen et al.
The Journal of clinical endocrinology and metabolism, 99(6), 2155-2163 (2014-03-13)
Observations in rodents suggest that osteocalcin (OC) participates in glucose metabolism. Based on human studies, it remains unclear whether circulating OC is simply a bone turnover marker (BTM) or also a mediator in interactions between the skeleton and glucose homeostasis.
Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome.
Kadowaki T
The Journal of Clinical Investigation (2006)
Adiponectin attenuates Ang ?-induced TGF?1 production in human mesangial cells via an AMPK-dependent pathway.
Tan M
Biotechnology and Applied Biochemistry (2015)
Effect of Exercise Training on Metabolic Homeostasis and Some Hemodynamics (Some Hepatic and Cardiovascular Functions) in Experimentally Induced Obesity
SolimanNA, et al.
journal of Obesity & WeightLoss Therapy , 8(2) (2018)

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