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Merck
CN

RAB0307

Sigma-Aldrich

Human IL-6 ELISA Kit

for cell and tissue lysates

Synonym(s):

B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 1.37-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL6(3569)

General description

The Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-6 cell lysate and tissue lysate.

Immunogen

Recombinant Human IL-6

Application

For research use only. Not for use in diagnostic procedures.
Human IL-6 ELISA kit has been used for determining the levels of IL-6 in human cells.

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Information

低风险生物材料
常规特殊物品
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Gaohua Han et al.
Oncology letters, 18(1), 321-329 (2019-07-11)
The present study evaluated the role of annexin A1 (ANXA1) in the treatment of acute radiation-induced lung injury (RILI) and investigated the mechanism of its action. The expression of ANXA1, interleukin-6 (IL-6) and myeloperoxidase (MPO) in the plasma of patients
Hongjun Yang
American journal of translational research, 13(7), 8391-8396 (2021-08-12)
To explore the cytokine expression level of interstitial lung disease in primary Sjogren's syndrome (ILD-pSS) patients and its clinical significance. 50 pSS patients in our hospital from Jan 2017 to Dec 2019 were selected as pSS group and separated into
Interleukin-6: discovery of a pleiotropic cytokine.
Kishimoto T
Arthritis Research & Therapy, 8, S2-S2 (2006)
Marietta Charakida et al.
The lancet. Diabetes & endocrinology, 2(8), 648-654 (2014-05-27)
Excess body fat is associated with an increase in risk of type 2 diabetes and hypertension in adulthood and these risks can adversely affect progression of arterial disease. We aimed to assess the impact of lifelong patterns of adiposity on
Suresh Gopi Kalathil et al.
American journal of respiratory and critical care medicine, 190(1), 40-50 (2014-05-16)
Previous studies from our laboratory have shown that peripheral blood mononuclear cells (PBMCs) from patients with chronic obstructive pulmonary disease (COPD) prone to exacerbations with nontypeable Haemophilus influenzae have impaired responses to lipoprotein P6. We hypothesized that an underlying immunosuppressive

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