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RES6008H-A7

HEPES, Hemisodium Salt

Synonym(s):

HEPES hemisodium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17.5N2O4S· 0.5Na
CAS Number:
103404-87-1
Molecular Weight:
249.30
NACRES:
NA.21
UNSPSC Code:
12352200

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InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

biological source

synthetic

assay

≥96.0% NaOH basis (Reverse titration)

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin and microbial; tested

useful pH range

6.8-8.2

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

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Application

HEPES Hemisodium is a biological buffer often referred to as a “Good′s” buffer. As the hemisodium salt, this buffer can be used directly to prepare a working buffer solution of HEPES. This biological buffer formulation is ideal since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Hemisodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES Hemisodium can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES6008H-A701X: 100 gm container
RES6008H-A702X: 1 kg container
RES6008H-A704X: 10 kg container

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
CDBF5455
CDBD6066
CDBD6064
CDBD6065
CDBD1884

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