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RIP

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Imprint® RNA Immunoprecipitation Kit

High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

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Synonym(s):
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
NACRES:
NA.54

Quality Level

200

Related Categories

General description

Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.

Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.

Application

Imprint® RNA Immunoprecipitation Kit has been used for:
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Xtra Protein A magnetic beads with higher binding capacity
  • Step-by-step protocols for cell lysis and immunoprecipitation
  • RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
  • Negative control and antibody included

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
  • R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLSDS

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Ammonium acetate solution, BioUltra, for molecular biology, ~5 M in H2O

Pictograms

FlameCorrosion
GHS02,GHS05

Signal Word

Danger

Hazard Statements

H226 - H315 - H318 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

100.4 °F

Flash Point(C)

38 °C

Regulatory Information

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Articles

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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