Sigma′s GenElute^™ Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). The kit can recover RNA from as little as a single cell to 2 x 10^5 cells. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Fast and easy processing using rapid micro spin-column format
• Small elution volume of 8 μL
• Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
• No phenol or chloroform extractions
• Isolate high quality total RNA from a variety of sources
• RNA can be isolated and detected from as little as a single animal cell

Preps

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

GenElute is a trademark of Sigma-Aldrich Co. LLC