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Sigma-Aldrich

Stemline® II Hematopoietic Stem Cell Expansion Medium

Serum-free, contains L-glutamine, liquid, sterile-filtered, suitable for cell culture

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NACRES:
NA.81

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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General description

The second generation of Sigma′s hematopoietic stem cell expansion media family, Stemline II has been developed to optimize the balance of differientiated and undifferentiated cells while maximizing their expansion. Compatible with Hematopoietic Stem Cells (HSC) from bone marrow, cord blood, and mobilized peripheral blood, Stemline II has been shown to lead to significant increases in cell expansion from all three sources. Through flow cytometric analysis of clinical-scale expansions, Stemline II has also demonstrated a higher capacity than other commercially available media for the expansion of CD34+/CD38+ late progenitors required for short-term engraftment. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline′s utility in true functional trial.

Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for your HSC applications.

Please click here to contact us if you are considering including the use of this product in an Investigational New Drug application.

Reconstitution

Must be supplemented with growth factors and cytokines according to individual user protocols.

Legal Information

Stemline is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Malgorzata Stec et al.
Journal of leukocyte biology, 82(3), 594-602 (2007-06-28)
To determine whether monocytes can be generated from CD34+ hematopoietic progenitors in large numbers, cord blood CD34+ cells were first expanded for 3-10 days in X-VIVO 10 medium supplemented with FCS, stem cell factor (SCF), thrombopoietin (TPO), and Flt-3 Ligand
Annika Wulf-Goldenberg et al.
European journal of cell biology, 87(2), 69-80 (2007-10-13)
Stem cell homing, engraftment and organ regeneration are controlled by cytokines, chemokines and cell-cell interactions. In this paper, cytokine effects on homing- and engraftment-related characteristics of CD34(+) cord blood cells were examined. Untreated CD34(+) cells were mainly in the G(0)/G(1)
Taira Nakayama et al.
PloS one, 14(1), e0210198-e0210198 (2019-01-27)
Various cell-based therapeutic strategies have been investigated for vascular and tissue regeneration after ischemic stroke. We have developed a novel cell population, called regeneration-associated cells (RACs), by quality- and quantity-controlled culture of unfractionated mononuclear cells. RACs were trans-arterially injected into
Shi-Jiang Lu et al.
Nature methods, 4(6), 501-509 (2007-05-09)
Recent evidence suggests the existence of progenitor cells in adult tissues that are capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Here we describe an efficient and reproducible method for generating large numbers of
Comparison of Serum-Free Media in RBC Differentiation from Human Hematopoietic Stem Cells
Kim, Ji Yeon. et al
Korean Journal of Blood Transfusion, 26, 18-25 (2015)
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Protocols

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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