S1062
2-O-Sulfatase from Flavobacterium heparinum
10-40 (units/ml)
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About This Item
MDL number:
UNSPSC Code:
12352204
form
solution
specific activity
10-40 (units/ml)
shipped in
dry ice
storage temp.
−20°C
General description
Hydrolyses the terminal 2-O-sulphate on the unsaturated di- and oligo-saccharides produced by the action of lyases on sulphated glycosaminoglycans.
Application
2-O-Sulfatase from Flavobacterium heparinum has been used in a study to determine that oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events. 2-O-Sulfatase from Flavobacterium heparinum has also been used in a study to determine the structure of oligosaccharides prepared from acharan sulfate.
Physical form
Solution contains 10 mM tris-acetate, pH 7.3, 80 mM NaCl, 10 mM potassium phosphate, 0.2% BSA.
Other Notes
One unit will hydrolyze heparin disaccharide I-P (H9401) to yield 1.0 micromole of disaccharide II-P (δ-UA-GlcNCOEt-6S) per minute at pH 7.3 at 25 °C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Y S Kim et al.
Glycobiology, 8(9), 869-877 (1998-07-24)
The fine structure of acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica , was examined. This glycosaminoglycan has a major disaccharide repeating unit of -->4)-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S(1--> (where GlcNpAc is N -acetylglucosamine, IdoAp is iduronic acid, and S is sulfate)
Marco Guerrini et al.
Nature biotechnology, 26(6), 669-675 (2008-04-26)
Recently, certain lots of heparin have been associated with an acute, rapid onset of serious side effects indicative of an allergic-type reaction. To identify potential causes for this sudden rise in side effects, we examined lots of heparin that correlated
Rahul Raman et al.
The Journal of biological chemistry, 278(14), 12167-12174 (2003-01-10)
In the previous paper (Myette, J. R., Shriver, Z., Claycamp, C., McLean, M. W., Venkataraman, G., and Sasisekharan, R. (2003) J. Biol. Chem. 278, 12157-12166), we described the molecular cloning, recombinant expression, and preliminary biochemical characterization of the heparin/heparan sulfate
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