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Merck
CN

S1569

Stemline® Mesenchymal Stem Cell Expansion Medium

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

MSC Medium

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About This Item

NACRES:
NA.81
UNSPSC Code:
12352200
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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Developed to promote the optimal expansion of human mesenchymal stem cells (MSC) from bone marrow, Stemline Mesenchymal Stem Cell Expansion Medium demonstrates greater total nucleated cell (TNC) fold increases than other commercially available formulations. Additionally, functional trials clearly demonstrated Stemline′s capacity to promote differentiation into adipocytes, chondrocytes, and osteocytes.

Stemline Mesenchymal Stem Cell Expansion Medium requires supplementation with antibiotics, cytokines, L-glutamine and fetal bovine serum, as appropriate to individual research protocols. Known to be extremely sensitive during initial isolation and growth ex vivo, MSC proliferation depends highly on the composition of fetal bovine serum (FBS) used to supplement their medium. Pre-screening with FBS is recommended, as the specific FBS components that effect MSC growth have not been fully identified.

Produced in a GMP state-of-the-art facility, Stemline Mesenchymal Stem Cell Expansion Medium is clearly an excellent choice for your MSC applications.

Preparation Note

Medium must be supplemented with antibiotics, cytokines, 4 mM L-glutamine, and fetal bovine serum according to individual user protocols.

Legal Information

Stemline is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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I McNiece et al.
Cytotherapy, 6(4), 311-317 (2005-09-09)
Cord blood (CB) cells are being used increasingly as a source of hematopoietic cells to support high dose chemotherapy. However, CB units contain low numbers of cells, including CD34+ cells, and thus their use is associated with significant delays in
J Rodrigo Mora et al.
Science (New York, N.Y.), 314(5802), 1157-1160 (2006-11-18)
Normal intestinal mucosa contains abundant immunoglobulin A (IgA)-secreting cells, which are generated from B cells in gut-associated lymphoid tissues (GALT). We show that dendritic cells (DC) from GALT induce T cell-independent expression of IgA and gut-homing receptors on B cells.

Articles

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

新型无血清、无异种人间充质干细胞(MSC)骨细胞分化培养基的开发。

Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

有关间充质的信息,特别是间充质干细胞培养实验方案用于间充质干细胞(MSC)分离、扩增和分化的分步细胞培养方案。

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