Stemline^® T Cell Expansion Medium

Stemline^® T Cell Expansion Medium has been used to generate silver (Ag)-specific T-cells.

Developed to promote the optimal expansion of adult human T cells, Stemline T Cell Expansion Medium demonstrates significantly greater expansion (55%) when compared to alternative media, and viability greater than 95%. Additionally, flow cytometry confirms that with Stemline, a proper CD4/CD8 ratio is maintained. In an ex vivo functional assay (51Chromium Release Assay), T cells expanded in Stemline medium proved to be highly functional and possessed cytolytic potential greater than T cells expanded in serum-containing medium (RPMI with 10% fetal bovine serum). In an in vivo functional assay (GvHD Induction), human T lymphocytes expaned in Stemline medium were injected into NOD/SCIDb2M mice (n=12). Engraftment, perivascular infiltration, and lethal GvHD were observed by day 15 in 100% of mice, demonstrating excellent in vivo expansion and functionality.

Stemline T Cell Expansion Medium is free of serum and all other animal-derived components with the exceptions of human serum albumin and transferrin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline T-Cell Expansion Medium is clearly an excellent choice for your T-cell applications.

The medium does not contain antibiotics or cytokines. Human serum albumin and transferrin are the only human origin material and are non-reactive (donor level) for anti-HIV 1 & 2, anti-HCV and HB_sAg.

Add 20 mL of 200 mM L-glutamine solution or 0.584 g of γ-irradiated L-glutamine powder per liter of solution.

Stemline is a registered trademark of Merck KGaA, Darmstadt, Germany