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Merck
CN

S2415

Streptavidin−Iron Oxide Particles from Streptomyces avidinii

buffered aqueous suspension, ~1.5 μm

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
Form:
buffered aqueous suspension
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form

buffered aqueous suspension

concentration

0.8-1.2 mg/mL

matrix

Superparamagnetic Iron Oxide Particles

matrix activation

amine

particle size

~1.5 μm

capacity

≥2 μg/mg binding capacity (poly(A) RNA)(using biotinylated oligo(dT)20)

foreign activity

DNase and RNase, none detected

storage temp.

2-8°C

Quality Level

Application

Streptavidin iron oxide particles are used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin iron oxide particles have been used to study imaging of cellular spread on a three-dimensional scaffold by means of a novel cell-labeling technique for high-resolution computed tomography. Streptavidin iron oxide particles have also been used to study in vitro biomedical applications of functionalized iron oxide nanoparticles.
Used for the purification of biotin containing proteins or DNA binding proteins

Physical form

Suspension in 0.85% NaCl, 0.01 M phosphate, pH 8.0, containing 0.1% bovine serum albumin, 0.1% sodium azide

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

含少量动物源组分生物产品
常规特殊物品
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Michaela Šiková et al.
The EMBO journal, 39(3), e102500-e102500 (2019-12-17)
RNase J1 is the major 5'-to-3' bacterial exoribonuclease. We demonstrate that in its absence, RNA polymerases (RNAPs) are redistributed on DNA, with increased RNAP occupancy on some genes without a parallel increase in transcriptional output. This suggests that some of
Saeed Taebi et al.
Journal of immunological methods, 458, 26-32 (2018-04-15)
Hepatitis B virus (HBV) infection is the major public health problem leading cause of death worldwide. The most important diagnostic marker for this infection is hepatitis B surface antigen (HBsAg). In this study, a novel, inexpensive, portable and sensitive ELISA
Paula Portela et al.
The Journal of biological chemistry, 277(34), 30477-30487 (2002-06-14)
Saccharomyces cerevisiae pyruvate kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA-Tpk1.Bcy1) and to be phosphorylated in a cAMP-dependent process. Both glutathione S-transferase (GST)-Pyk1 and GST-Pyk2 were phosphorylated in vitro by
Rahul Gaur et al.
Journal of bacteriology, 187(20), 6893-6901 (2005-10-04)
Queuosine (Q), one of the most complex modifications occurring at the wobble position of tRNAs with GUN anticodons, is implicated in a number of biological activities, including accuracy of decoding, virulence, and cellular differentiation. Despite these important implications, its biosynthetic
Benjamin W Thimm et al.
Tissue engineering. Part C, Methods, 18(3), 167-175 (2011-09-29)
Computed tomography (CT) represents a truly three-dimensional (3D) imaging technique that can provide high-resolution images on the cellular level. Thus, one approach to detect single cells is X-ray absorption-based CT, where cells are labeled with a dense, opaque material providing

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