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Merck
CN

S3938

Sigma-Aldrich

SeqSaver Sequencing Premix Dilution Buffer

for DNA sequencing

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25
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grade

Molecular Biology

form

liquid

storage temp.

−20°C

Application

Suitable for use with the ABI BigDye® terminator premix solution for sequencing.

Features and Benefits

  • Reduces the amount of Terminator Premix required
  • Compatable with BigDye®, DYEnamic ETs, ThermoSequemase II
  • No reduction in resolution, read length, or signal strength
  • Compatable with ABI Prism 3700, 3100 & 310 DNA sequencers
  • Also compatable with Molecular Dynamics MegaBACE instruments

Legal Information

BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqSaver is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Information

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Identification of SNPs, or Mutations in Sequence Chromatograms
Draper N
Meths. Mol. Biol., 439, 35-52 (2008)
Vivek S Yellore et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 9(4), 228-234 (2007-04-18)
The study purpose was to identify the genetic basis of posterior polymorphous corneal dystrophy, an autosomal dominant disorder of the corneal endothelium that is associated with the development of corneal edema, necessitating corneal transplantation for visual rehabilitation. Glaucoma also develops
L Wen
Molecular biotechnology, 17(2), 135-142 (2001-06-09)
The use of automated fluorescent DNA sequencer systems and PCR-based DNA sequencing methods plays an important role in the actual effort to improve the efficiency of large-scale DNA analysis. While dideoxy-terminators labeled with energy-transfer dyes (BigDyes) provide the most versatile
Anthony J Aldave et al.
Investigative ophthalmology & visual science, 51(8), 4006-4012 (2010-04-02)
To identify the genetic basis of posterior amorphous corneal dystrophy (PACD) segregating in a large pedigree. The authors performed clinical evaluation of a previously unreported pedigree with PACD, light and electron microscopic examination of an excised corneal button, genomewide linkage
Anthony J Aldave et al.
Cornea, 28(7), 801-807 (2009-07-04)
Posterior polymorphous corneal dystrophy (PPCD) is an autosomal-dominant disorder of the corneal endothelium associated with visually significant corneal edema and glaucoma. Statistical genetic analysis of 4 families with PPCD has demonstrated linkage to a 2.4 cM common support interval on

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