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S7651

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
9031-54-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-869-9
MDL number:
MFCD00132362
EC Number:
3.1.4.12 (BRENDA, IUBMB)
Specific activity:
≥100 units/mg protein
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form

lyophilized powder

Quality Segment

200

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

General description

Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.
Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices
  • for testing and confirming its silkworm-killing activity
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

wgk

WGK 1

Hazard Classifications

Resp. Sens. 1

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

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