S9319
Anti-α/β-SNAP (140-157) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, rat, mouse
technique(s)
western blot: 1:1,000-1:2,000 using cytosolic fraction S1 of rat and mouse brain
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NAPA(8775), NAPB(63908)
mouse ... Napa(108124)
rat ... Napa(140673)
General description
α-SNAP is 80% homologous to β-SNAP and is expressed in all mammalian tissues, whereas β-SNAP is expressed only in brain.
αSNAP (α-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) is widely present and abundantly expressed in all tissues. It is located on human chromosome 19q13.32. βSNAP (β-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) is expressed only in neuronal tissues and is located on human chromosome 20p11.21.
Immunogen
synthetic peptide encoding amino acids 140-157 located near the mid-region of mouse α-SNAP, conjugated to KLH. The sequence is identical in human, rat, and bovine α-SNAP and in human, mouse, rat and bovine β-SNAP.
Application
Anti-α/β-SNAP (140-157) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
α-SNAP binding to the soluble NSF attachment protein receptor (SNARE) complex, recruits NSF to form a 20S complex. ATP hydrolysis by NSF ATPase activity induces disassembly of the 20S complex, thus facilitating vesicle fusion to the membrane and exocytosis.
α-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein (α-SNAP) is an essential constituent of the membrane fusion machinery. It plays an important role in insulin exocytosis through large dense core vesicles. In human epithelia, absence of αSNAP promotes non-canonical autophagy. α-SNAP and NSF are highly essential in the initial stage of human sperm acrosome reaction.
Anti-α/β-SNAP (KS-18) recognizes α/β-SNAP.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Molecular cytogenetic characterization of an inherited maternal duplication 20p11. 21p13 associated with a small 20p11. 21 deletion.
D'angelo CS, et al.
American Journal of Medical Genetics. Part A, 152(12), 3197-3202 (2010)
α-SNAP and NSF are required in a priming step during the human sperm acrosome reaction.
Tomes CN, et al.
Molecular Human Reproduction, 11(1), 43-51 (2005)
19q13. 32 microdeletion syndrome: three new cases.
Castillo A, et al.
European Journal of Medical Genetics, 57(11), 654-658 (2014)
Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes.
Prekeris R, et al.
The Journal of Cell Biology, 143(4), 957-971 (1998)
α-Soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic β cells and functions in insulin but not γ-aminobutyric acid secretion.
Nagamatsu S, et al.
The Journal of Biological Chemistry, 274(12), 8053-8060 (1999)
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