S9511
Superoxide Dismutase from canine erythrocytes
lyophilized powder, 2,000-6,000 units/mg protein
Synonym(s):
SOD, Superoxide: superoxide oxidoreductase
form
lyophilized powder
specific activity
2,000-6,000 units/mg protein
mol wt
~31,200 (two identical subunits)
composition
Protein, ≥90% biuret
UniProt accession no.
storage temp.
−20°C
Gene Information
dog ... SOD1(403559)
Biochem/physiol Actions
Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby promoting the activity of NO.1 SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines and transgenic mice.2,3
Physical form
Lyophilized powder containing potassium phosphate buffer salts
Analysis Note
For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
Other Notes
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Bovine erythrocyte superoxide dismutase. Complete amino acid sequence.
H M Steinman et al.
The Journal of biological chemistry, 249(22), 7326-7338 (1974-11-25)
Ou-Yang Chao et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 68(1-2), 60-69 (2013-05-11)
We report cDNA cloning, expression, purification, and characterization of a novel Cu/ Zn superoxide dismutase (SOD) from Jatropha curcas leaves. The full-length cDNA of this SOD contained a 496-bp open-reading frame (ORF) encoding 162 amino acid residues. The recombinant plasmid
Raymond S W Tsang et al.
Canadian journal of microbiology, 59(5), 362-364 (2013-05-08)
Haemophilus influenzae serotype a (Hia) is an important pathogen since the introduction of vaccines for control of disease due to serotype b strains. Using a sodC-based polymerase chain reaction, Hia can be divided into 2 phylogenetic divisions, each with their
Jian-Yu Liu et al.
Zhen ci yan jiu = Acupuncture research, 38(1), 48-51 (2013-05-09)
To observe the effect of electroacupuncture (EA) intervention on free radical metabolism in athletes during intensive endurance exercise, so as to reveal its mechanism underlying fatigue relief. Thirty male college student volunteers were equally divided into control group, intensive endurance
From mice to men: gene therapy's future for treatment of myocardial infarction.
Brian G Kral et al.
Circulation. Cardiovascular imaging, 6(3), 360-362 (2013-05-23)
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