S9751
Sulfatase from Helix pomatia
Type H-2, aqueous solution, ≥2,000 units/mL
Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
type
Type H-2
Quality Level
form
aqueous solution
secondary activity
≥100,000 units/mL β-glucuronidase (at pH 5.0)
mol wt
~85 kDa by gel filtration
concentration
≥2,000 units/mL
shipped in
wet ice
storage temp.
2-8°C
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General description
Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.
Application
Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
低风险生物材料
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Fernando Cámara-Martos et al.
Foods (Basel, Switzerland), 11(3) (2022-02-16)
In northwest Spain and Portugal, there is a long tradition of cultivating B. rapa subsp. rapa to obtain turnip greens and turnip tops. Brassica rapa L. subsp. rapa (turnip greens and turnip tops) were grown under conventional and organic conditions
Tania Merinas-Amo et al.
Foods (Basel, Switzerland), 10(11) (2021-11-28)
Brassica rapa L. subsp. rapa (turnip greens), a traditionally consumed vegetable, is well-known due to its high content of glucosinolates, which are secondary metabolites with a positive biological activity for human health. Our hypothesis has been based on the relation
Abigail M Tokheim et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 18(5), 537-540 (2005-12-08)
Often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from Helix pomatia remain little characterized. As many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the
Jakub Kopycki et al.
Biotechnology letters, 33(5), 1039-1046 (2011-01-27)
Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action
Monica L Vermillion Maier et al.
Drug metabolism and disposition: the biological fate of chemicals, 49(8), 694-705 (2021-05-27)
3,3'-Diindolylmethane (DIM), a major phytochemical derived from ingestion of cruciferous vegetables, is also a dietary supplement. In preclinical models, DIM is an effective cancer chemopreventive agent and has been studied in a number of clinical trials. Previous pharmacokinetic studies in
Protocols
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
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