Anti-rde-1 (center) antibody produced in rabbit

Double-stranded (ds) RNA is a potent sequence-specific inhibitor of gene function. RNA interference (RNAi) is a cellular defense mechanism that uses double-stranded RNA (dsRNA) as a sequence-specific trigger to guide the degradation of homologous single-stranded RNAs. RNAi is a multistep process involving several proteins and at least one type of RNA intermediate, a population of small 21–25 nt RNAs (called siRNAs) that are initially derived from cleavage of the dsRNA trigger. Genetic studies have implicated several RNA interference-deficient (rde) family members in germline maintenance and development, and several simple loss of function mutants have been identified. Family members rde-1 and rde-4 are required for RNAi but are not essential for organismal viability. While rde-1 and rde-4 are distinct from other RNAi-deficient family members both both for their inability to mobilize transposons and lack of chromosome loss, each appears to have a distinct role in the interference mechanism. Evidence indicates that rde-4 is involved before or during production of siRNAs, whereas rde-1 acts after the siRNAs have been formed.

RDE (NP_741611, )
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of C. elegans Rde-1.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.