SAB1306078
ANTI-FLAG TAG (DYKDDDDK) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
all
concentration
(see Certificate of Analysis)
technique(s)
western blot: 1:1000
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
FLAG is an octapeptide containing the following amino acid sequence Asp-Try-Lys-Asp-Asp-Asp-Asp-Lys.
Application
ANTI-FLAG TAG (DYKDDDDK) antibody produced in rabbit has been used in western blotting, (1:5000), (1:10000)
Biochem/physiol Actions
FLAG is a widely used epitope tag for immunoaffinity purification of fusion proteins.
Physical form
Supplied in PBS with 0.09% (W/V) sodium azide
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Sunwang Xu et al.
Nature communications, 10(1), 5492-5492 (2019-12-04)
Gemcitabine is the first-line treatment for locally advanced and metastatic gallbladder cancer (GBC), but poor gemcitabine response is universal. Here, we utilize a genome-wide CRISPR screen to identify that loss of ELP5 reduces the gemcitabine-induced apoptosis in GBC cells in
Renxu Chang et al.
Nature communications, 9(1), 3486-3486 (2018-08-30)
Loss of WW domain-containing oxidoreductase (Wwox) expression has been observed in breast cancer (BC). However, its regulatory effects are largely unknown, especially in triple-negative breast cancer (TNBC). Herein, gene expression profiling revealed that JAK/STAT3 pathway was one of the most
Feiran Zhang et al.
Human molecular genetics, 27(22), 3936-3950 (2018-08-15)
N6-methyladenosine (m6A) is the most prevalent internal modification of mammalian messenger RNAs (mRNAs) and long non-coding RNAs. The biological functions of this reversible RNA modification can be interpreted by cytoplasmic and nuclear 'm6A reader' proteins to fine-tune gene expression, such
Yajie Gao et al.
STAR protocols, 3(3), 101596-101596 (2022-08-06)
Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation of RNA probes, the target
B L Brizzard et al.
BioTechniques, 16(4), 730-735 (1994-04-01)
The FLAG epitope is an eight amino acid peptide (AspTyrLysAspAspAspAspLys) that is useful for immunoaffinity purification of fusion proteins. A monoclonal antibody (anti-FLAG M1) that binds the FLAG epitope in a calcium-dependent manner and requires an N-terminal FLAG sequence has
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