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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GT359, monoclonal
Application:
ELISA, IF, IP, WB
Citations:
15
biological source
mouse
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
GT359, monoclonal
form
buffered aqueous solution
species reactivity
human, mouse
concentration
1mg/mL
technique(s)
ELISA: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: 500-3000
isotype
IgG3
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.
Immunogen
The immunogen used to generate this antibody corresponds to 6X His tag
Application
Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.
Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.
Biochem/physiol Actions
6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Phosphate-buffered saline, no preservative added.
Other Notes
Purification: Affinity purified by Protein G
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
常规特殊物品
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Jordan Demone et al.
PloS one, 17(12), e0277668-e0277668 (2022-12-15)
Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield
Erratum: Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase.
Frontiers Production Office
Frontiers in Bioengineering and Biotechnology, 10, 878674-878674 (2022)
SKP2-and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Yao F, et al.
Nature Communications, 9(1), 2269-2269 (2018)
James S Terry et al.
Virology, 558, 28-37 (2021-03-15)
To help fight COVID-19, new molecular tools specifically targeting critical components of the causative agent of COVID-19, SARS-Coronavirus-2 (SARS-CoV-2), are desperately needed. The SARS-CoV-2 nucleocapsid protein is critical for viral replication, integral to viral particle assembly, and a major diagnostic
Disrupting CD147-RAP2 interaction abrogates erythrocyte invasion by Plasmodium falciparum.
Z Meng-Yao, et al.
Blood, 131(10), 1111-1121 (2018)
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