SAB2702434
Anti-PU.1/Spi1 antibody produced in rabbit
affinity isolated antibody
Synonym(s):
cb1086, pu.1, pu1, sfpi1
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
34kDa
species reactivity
zebrafish
concentration
1mg/mL
technique(s)
immunohistochemistry: suitable
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Gene Information
zebrafish ... spi1b(30117)
General description
PU.1 is a transcription factor that belongs to the erythroblast transformation specific (ETS) family. This protein is highly expressed in hematopoietic cells like mast cells, B lymphocytes, neutrophils, early erythroblasts, and macrophages. PU.1 protein consists of a highly conserved DNA binding domain, a glutamine-rich domain, acidic residues towards the N-terminal, and a central proline-glutamic acid-serine-threonine (PEST) domain.
Immunogen
Recombinant protein encompassing a sequence within the center region of zebrafish PU.1/Spi1.
Application
Suggested starting dilutions are as follows: IHC-Wm: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Biochem/physiol Actions
PU.1 transcription factor facilitates the regulation of myeloid differentiation. This transcription factor is also involved in regulating several other myeloid genes such as macrophage colony-stimulating factor (M-CSF) receptor, CD11 antigen-like family member B (CD11B), granulocyte colony-stimulating factor (G-CSF) receptor, and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor. Dysregulation of the PU.1 gene results in an increase in erythroblast proliferation, inhibits erythroid differentiation and leads to erythroleukemia.
Features and Benefits
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Physical form
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Other Notes
Purification: Purified by antigen-affinity chromatography
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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P Zhang et al.
Blood, 96(8), 2641-2648 (2000-10-07)
The lineage-specific transcription factors GATA-1 and PU.1 can physically interact to inhibit each other's function, but the mechanism of repression of GATA-1 function by PU.1 has not been elucidated. Both the N terminus and the C terminus of PU.1 can
S R McKercher et al.
The EMBO journal, 15(20), 5647-5658 (1996-10-15)
PU.1 is a member of the ets family of transcription factors and is expressed exclusively in cells of the hematopoietic lineage. Mice homozygous for a disruption in the PU.1 DNA binding domain are born alive but die of severe septicemia
Beatrice U Mueller et al.
Blood, 100(3), 998-1007 (2002-07-20)
The transcription factor PU.1 is required for normal blood cell development. PU.1 regulates the expression of a number of crucial myeloid genes, such as the macrophage colony-stimulating factor (M-CSF) receptor, the granulocyte colony-stimulating factor (G-CSF) receptor, and the granulocyte-macrophage colony-stimulating
C Nerlov et al.
Blood, 95(8), 2543-2551 (2001-02-07)
The GATA-1 transcription factor is capable of suppressing the myeloid gene expression program when ectopically expressed in myeloid cells. We examined the ability of GATA-1 to repress the expression and function of the PU.1 transcription factor, a central regulator of
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