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SAB4200090

Anti-Raver1 (C-terminal) antibody produced in rabbit

IgA fraction of antiserum

Synonym(s):

Anti-Ribonucleoprotein PTB-binding 1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
3
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biological source

rabbit

conjugate

unconjugated

antibody form

IgA fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~77 kDa

technique(s)

immunoprecipitation (IP): 5-10 μL using lysates of HEK-293Tcells overexpressing human Raver1, indirect immunofluorescence: 1:500-1:1000 using paraformaldehyde fixed HEK-293T cells overexpressing human Raver1, western blot: 1:500-1:1000 using lysates of HEK-293T cells overexpressing human Raver1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAVER1(125950)
mouse ... Raver1(71766)
rat ... Raver1(298705)

General description

Raver1 is encoded by the gene mapped to human chromosome 19p13.2.
Raver1, also known as ribonucleoprotein phosphotyrosine-binding (PTB)-1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and both nuclear localization and nuclear export signals. It thus, shuttles between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI, a protein involved in RNA splicing of microfilament proteins.

Application

Anti-Raver1 (C-terminal) antibody produced in rabbit is suitable for:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Anti-Raver1 (C-terminal) recognizes human Raver1.
Variation in the raver1 gene expression may be associated with the development of depressive disorder. In skeletal muscle, migration of of Raver1 from the nucleus to the cytoplasm facilitates differentiation of specific microfilament attachment sites.
Vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Hee-Ju Kang et al.
Scientific reports, 10(1), 9927-9927 (2020-06-20)
The prevalence and clinical characteristics of depressive disorders differ between women and men; however, the genetic contribution to sex differences in depressive disorders has not been elucidated. To evaluate sex-specific differences in the genetic architecture of depression, whole exome sequencing
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
Huttelmaier S, et al.
The Journal of Cell Biology, 155(5), 775-786 (2001)
Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
Lee JH, et al.
Structure, 17(6), 833-842 (2009)

Global Trade Item Number

SKUGTIN
SAB4200090-200UL04061835686933
SAB4200090-25UL04061835686940