Key Documents

View All Documentation

SAB4200114

Anti-EDC4 (N-terminal) antibody produced in rabbit

Name: Anti-EDC4 (N-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Ge-1, Anti-HEDLS (Human enhancer of decapping, (large subunit), Anti-RCD-8

Select a Size

Change View

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using ysates of HEK-293 cells over expressing human EDC4, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing human EDC4, western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4

UniProt accession no.

Q6P2E9

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EDC4(23644)
mouse ... Edc4(234699)
rat ... Edc4(361399)

Description

General description

Enhancer of mRNA-decapping protein 4 (EDC4), also known as human enhancer of decapping large subunit (HEDLS) or Ge-1, is characterized with an N-terminal WD40 repeat and a C-terminal domain which interacts with mRNA-decapping enzyme subunit 2 (Dcp2), and mediates EDC4 oligomerization and P-body localization .

The EDC4 gene maps on human chromosome 16q22.1.

Application

Anti-EDC4 (N-terminal) antibody has been used in:

immunoprecipitation
immunofluorescence
immunoblotting.

Biochem/physiol Actions

Anti-EDC4 (N-terminal) specifically recognizes human EDC4.

Human enhancer of mRNA-decapping protein 4 (EDC4) associates with hDcp2 and stimulates hDcp2 activity in vitro as well as mediating the interaction between mRNA-decapping enzyme subunit 1 & 2 (DCP1 and DCP2).

Interaction of processing-body components, EDC4 and Dcp1a is essential for the post-transcriptional regulation of interleukin (IL)-6.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

030M4753

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKU	GTIN
SAB4200114-25UL	04061838034717
SAB4200114-200UL	04061838034700