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Merck
CN

SAB4200134

Sigma-Aldrich

Anti-PNK antibody ,Mouse monoclonal

clone H101.5, purified from hybridoma cell culture

Synonym(s):

Anti-Bifunctional polynucleotide phosphatase/kinase, Anti-DNA 5′-kinase/3′-phosphatase, Anti-PNKP, Anti-Polynucleotide kinase 3′-phosphatase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

H101.5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

monkey, human, bovine

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

western blot: 2.5-5.0 μg/mL using whole extracts of A549 cells

isotype

IgG1

UniProt accession no.

Q96T60

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PNKP(11284)

General description

Monoclonal Anti-PNK (mouse IgG1 isotype) is derived from the hybridoma H101.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human polynucleotide kinase 3′-phosphatase (PNK) recombinant protein. PNK comprises of a central phosphatase domain that is flanked by an N-terminal forkhead-associated (FHA) domain and a C-terminal kinase domain on either sides.

Immunogen

human PNK recombinant protein.

Application

Monoclonal Anti-PNK antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

PNK is a mammalian DNA repair enzyme which catalyzes the phosphorylation of DNA 5′-hydroxyl termini and the dephosphorylation of 3′-phosphate termini. PNK is involved in the single-strand break repair pathway and the non-homologous end joining pathway for double strand break repair. Mutations in PNK result in neurological abnormalities.
Polynucleotide kinase 3′-phosphatase (PNKP or PNK) is a nuclear protein that repairs DNA strand breaks induced by oxidative damage or ionizing radiation. Genetic alterations in PNKP have been linked to seizures, microcephaly and deficiencies in DNA repair mechanisms .

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
040M4764

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Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase
Fanta M, et al.
Hybridoma, 20(4), 237-242 (2001)
Human polynucleotide kinase participates in repair of DNA double-strand breaks by nonhomologous end joining but not homologous recombination
Karimi BF, et al.
Cancer research, 67(14), 6619-6625 (2007)
PNKP knockdown by RNA interference inhibits herpes simplex virus-1 replication in astrocytes
Yue L, et al.
Virologica Sinica, 28(6), 345-351 (2013)
F Karimi-Busheri et al.
The Journal of biological chemistry, 274(34), 24187-24194 (1999-08-14)
Human polydeoxyribonucleotide kinase is an enzyme that has the capacity to phosphorylate DNA at 5'-hydroxyl termini and dephosphorylate 3'-phosphate termini and, therefore, can be considered a putative DNA repair enzyme. The enzyme was purified from HeLa cells. Amino acid sequence
N K Bernstein et al.
Anti-cancer agents in medicinal chemistry, 8(4), 358-367 (2008-05-14)
The cytotoxicity of many antineoplastic agents is due to their capacity to damage DNA and there is evidence indicating that DNA repair contributes to the cellular resistance to such agents. DNA strand breaks constitute a significant proportion of the lesions
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