Anti-Synemin (C-terminal region) antibody produced in rabbit

Synemin exists in two isoforms, α and β.

Synemin is a 230kDa intermediate filament protein (IFp). It is expressed along with desmin and vimentin filaments in muscle cells. In mouse, synemin is encoded by the gene mapped in B5 region of chromosome 7. Alternative splicing of this gene produces three different isoforms with different molecular mass namely, high (180kDa), medium (150kDa) and low (41kDa). High and medium isoforms are expressed in embryonic mouse, whereas, low molecular weight protein is expressed in adult mouse. Synemin comprises ∼310-amino acid rod domain characteristic of IF proteins and 1,290 amino acid tail domain at its C- terminal.

Anti-Synemin (C-terminal region) antibody produced in rabbit is suitable for immunoblotting.

α-Synemin is essential for the stabilization of junctional complexes at sarcolemma, in neonates. β-Synemin controls the connection of desmin with Z-disks. Synemin is also involved in protein phosphorylation, for regulating key signaling cascades in the heart. Mild myopathy has been observed in synemin knockout mice.

Anti-Synemin (C-terminal region) specifically recognizes rat synemin.

Synemin in association with either desmin or vimentin plays a vital role in cytoskeletal organization and also facilitates function of intermediate filaments in erythrocytes and muscle. Interaction of synemin and vinculin aids in attachment of intermediate filaments (Ifs) to the cell membrane.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Store at -20 °C.For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation.Working dilutions should be discarded if not used within 12 hours.

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