Anti-SORBS3 antibody, Mouse monoclonal

Monoclonal Anti-SORBS3 (mouse IgG2b isotype) is derived from the hybridoma SORB84 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The gene for sorbin and SH3 domain containing 3 (SORBS3) (also known as vinexin) protein is mapped to human chromosome 8p21.3. This protein belongs to the family of adaptor proteins. All the family members share a sorbin homology (SoHo) domain in the N-terminal region and three Src homology 3 (SH3) domains in the C-terminal region. There are three different isoforms of SORBS3 (α, β, and γ) protein that has been characterized. Isoform α and β contain three SH3 domains while the β isoform does not include the N-terminal SoHo domain. The γ isoform of vinexin has been characterized in mouse fetal gonads. It differs from the α isoform in a few amino acids that are missing from the N-terminal region. Vinexin ⓰ is ubiquitously expressed but vinexin α and γ show tissue-specific expression.

Anti-SORBS3 antibody, Mouse monoclonal is suitable for immunoblotting.

Monoclonal Anti-SORBS3 recognizes human, monkey, and rat SORBS3.

Vinexin α and β participates in cell signaling and the cytoskeletal structure. The α isoform is known to induce actin stress fiber formation and the β isoform plays a role in cell spreading. Vinexin might enhance the activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) in response to epidermal growth factor (EGF) stimulation by using its third SH3 domain. The growth-factor-induced signal transductions are controlled by the vinexin-son of sevenless (SOS) interactions. The sorbin homology (SoHo) domain has been shown to bind the lipid raft-associated protein flotillin1. The Src homology 3 (SH3) domain allows the binding of specific signaling and cytoskeletal molecules, including vinculin.

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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