SAB4200187
Anti-CACYBP antibody,Mouse monoclonal
clone CABP7, purified from hybridoma cell culture
Synonym(s):
Anti-GIG5, Anti-PNAS-107, Anti-RP1-102G20.6, Anti-S100A6BP, Anti-SIAH1-Interacting protein, Anti-SIP, Anti-calcyclin binding protein
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About This Item
Conjugate:
unconjugated
Clone:
CABP7, monoclonal
Application:
WB
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CABP7, monoclonal
form
buffered aqueous solution
mol wt
~27 kDa
species reactivity
canine, monkey, human, bovine
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
western blot: 2-4 μg/mL using SH-SY5Y, HeLa, COS-7 and MDCK total cell extracts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CACYBP(27101)
General description
Monoclonal Anti-CACYBP (mouse IgG1 isotype) is derived from the hybridoma CABP7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein. The protein is a human ortholog of mouse calcyclin (S100A6)-binding protein and an element of novel ubiquitin ligase complex.
The gene CACYBP (calcyclin binding protein) is mapped to human chromosome 1q25.1. It is widely expressed in human tissues.
Application
Anti-CACYBP antibody, Mouse monoclonal is suitable for immunoblotting.
Biochem/physiol Actions
CACYBP (calcyclin binding protein) is a part of the ubiquitination machinery and is involved in the degradation of β-catenin. It can interact with S100, SIAH1 (seven in absentia homolog 1) and SKP1 (S-phase kinase-associated protein 1). CACYBP is involved in proliferation, differentiation, multidrug resistance and tumorigenicity. It can work as an oncogene and as a tumor suppressor. In glioma cells, CACYBP enhances the cellular proliferation.
Monoclonal Anti-CACYBP recognizes human, dog, bovine, and monkey CACYBP.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Anna Filipek et al.
The Journal of biological chemistry, 277(32), 28848-28852 (2002-06-04)
Recently, a human ortholog of mouse calcyclin (S100A6)-binding protein (CacyBP) called SIP (Siah-1-interacting protein) was shown to be a component of a novel ubiquitinylation pathway regulating beta-catenin degradation (Matsuzawa, S., and Reed, J. C. (2001) Mol. Cell 7, 915-926). In
Genetic aberrations in prostate cancer by microarray analysis.
Saramaki OR, et al.
International Journal of Cancer. Journal International Du Cancer, 119, 1322-1329 (2006)
Xiaoxuan Ning et al.
Molecular cancer research : MCR, 5(12), 1254-1262 (2008-01-04)
Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P, has been identified as a component of a novel ubiquitinylation complex leading to beta-catenin degradation. However, the function of CacyBP/SIP in
CacyBP/SIP inhibits Doxourbicin-induced apoptosis of glioma cells due to activation of ERK1/2.
Tang Y, et al.
IUBMB Life, 68, 211-219 (2016)
Expression and regulation of CacyBP/SIP in chronic lymphocytic leukemia cell balances of cell proliferation with apoptosis.
Fu C, et al.
Journal of Cancer Research and Clinical Oncology, 142, 741-748 (2016)
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