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Merck
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SAB4200187

Sigma-Aldrich

Anti-CACYBP antibody,Mouse monoclonal

clone CABP7, purified from hybridoma cell culture

Synonym(s):

Anti-GIG5, Anti-PNAS-107, Anti-RP1-102G20.6, Anti-S100A6BP, Anti-SIAH1-Interacting protein, Anti-SIP, Anti-calcyclin binding protein

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About This Item

MDL number:
MFCD04040491
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CABP7, monoclonal

form

buffered aqueous solution

mol wt

~27 kDa

species reactivity

canine, monkey, human, bovine

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

western blot: 2-4 μg/mL using SH-SY5Y, HeLa, COS-7 and MDCK total cell extracts

isotype

IgG1

UniProt accession no.

Q9HB71

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CACYBP(27101)

General description

Monoclonal Anti-CACYBP (mouse IgG1 isotype) is derived from the hybridoma CABP7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein. The protein is a human ortholog of mouse calcyclin (S100A6)-binding protein and an element of novel ubiquitin ligase complex.
The gene CACYBP (calcyclin binding protein) is mapped to human chromosome 1q25.1. It is widely expressed in human tissues.

Application

Anti-CACYBP antibody, Mouse monoclonal is suitable for immunoblotting.

Biochem/physiol Actions

CACYBP (calcyclin binding protein) is a part of the ubiquitination machinery and is involved in the degradation of β-catenin. It can interact with S100, SIAH1 (seven in absentia homolog 1) and SKP1 (S-phase kinase-associated protein 1). CACYBP is involved in proliferation, differentiation, multidrug resistance and tumorigenicity. It can work as an oncogene and as a tumor suppressor. In glioma cells, CACYBP enhances the cellular proliferation.
Monoclonal Anti-CACYBP recognizes human, dog, bovine, and monkey CACYBP.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
070M4780

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Anna Filipek et al.
The Journal of biological chemistry, 277(32), 28848-28852 (2002-06-04)
Recently, a human ortholog of mouse calcyclin (S100A6)-binding protein (CacyBP) called SIP (Siah-1-interacting protein) was shown to be a component of a novel ubiquitinylation pathway regulating beta-catenin degradation (Matsuzawa, S., and Reed, J. C. (2001) Mol. Cell 7, 915-926). In
Genetic aberrations in prostate cancer by microarray analysis.
Saramaki OR, et al.
International Journal of Cancer. Journal International Du Cancer, 119, 1322-1329 (2006)
Xiaoxuan Ning et al.
Molecular cancer research : MCR, 5(12), 1254-1262 (2008-01-04)
Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P, has been identified as a component of a novel ubiquitinylation complex leading to beta-catenin degradation. However, the function of CacyBP/SIP in
CacyBP/SIP protein is important for the proliferation of human glioma cells.
Shi H, et al.
IUBMB Life, 66, 286-291 (2014)
CacyBP/SIP inhibits Doxourbicin-induced apoptosis of glioma cells due to activation of ERK1/2.
Tang Y, et al.
IUBMB Life, 68, 211-219 (2016)
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