SAB4200265
Anti-PTPA/ PPP2R4 antibody, Mouse monoclonal
clone 5G3, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-PP2A, Monoclonal Anti-PR53, Monoclonal Anti-phosphotyrosyl phosphatase activator, Monoclonal Anti-protein phosphatase 2A activator, regulatory subunit 4, Monoclonal Anti-protein phosphatase 2A, regulatory subunit B′ (PR 53)
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
5G3, monoclonal
form
buffered aqueous solution
mol wt
antigen 38 kDa
species reactivity
human, mouse
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using whole extracts of mouse 3T3 or human HeLa cells.
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PPP2R4(5524)
mouse ... Ppp2r4(110854)
rat ... Ppp2r4(362102)
General description
Monoclonal Anti-PTPA/ PPP2R4 (mouse IgG2a isotype) is derived from the hybridoma 5G3 produced by the fusion of mouse myeloma cells and splenocytes from the immunized BALB/c mice. Phosphotyrosyl phosphatase activator (PTPA), also known as protein phosphatase 2A activator, regulatory subunit 4 (PPP2R4), is a conserved protein from yeast to human. PTPA belongs to the B′ family and encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. The PTPA gene is mapped to human chromosome 9q34. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen
recombinant mouse PPP2R4, fusion protein
Application
Anti-PTPA/ PPP2R4 antibody, Mouse monoclonal is suitable for immunoblotting.
Monoclonal Anti-PTPA/ PPP2R4 antibody is suitable for use in western blot (0.5-1.0 μg/mL using whole extracts of mouse 3T3 or human HeLa cells).
Biochem/physiol Actions
Monoclonal Anti-PTPA/ PPP2R4 recognizes mouse and human PTPA/PPP2R4.
Phosphotyrosyl phosphatase activator (PTPA) is essential for the biogenesis of specific and active protein phosphatase 2A (PP2A). This protein acts as a peptidyl prolyl isomerase. It is known to promote the tyrosyl phosphatase activity of protein phosphatase 2A core heterodimer (PP2AD). PTPA regulates the PP2A-like enzymes. Deletion of the gene is considered lethal.
Protein phosphatase 2 phosphatase Activator (PTPA) is a protein phosphatase 2A (PP2A)-binding protein that activates the tyrosyl phosphatase function of PP2A and decreases the phosphoserine phosphatase function. Increased expression of PTPA has been linked to caspase-3 dependent cell death .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Nicolas Leulliot et al.
Molecular cell, 23(3), 413-424 (2006-08-04)
PTPA, an essential and specific activator of protein phosphatase 2A (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all alpha-helical
Jan Jordens et al.
The Journal of biological chemistry, 281(10), 6349-6357 (2005-12-29)
The protein phosphatase 2A (PP2A) phosphatase activator (PTPA) is an essential protein involved in the regulation of PP2A and the PP2A-like enzymes. In this study we demonstrate that PTPA and its yeast homologues Ypa1 and Ypa2 can induce a conformational
Thomas Fellner et al.
Genes & development, 17(17), 2138-2150 (2003-09-04)
Protein phosphatase 2A (PP2A) is an essential intracellular serine/threonine phosphatase containing a catalytic subunit that possesses the potential to dephosphorylate promiscuously tyrosine-phosphorylated substrates in vitro. How PP2A acquires its intracellular specificity and activity for serine/threonine-phosphorylated substrates is unknown. Here we
X Cayla et al.
The Journal of biological chemistry, 269(22), 15668-15675 (1994-06-03)
PTPA, or phosphotyrosyl phosphatase activator, is a protein that stimulates the tyrosyl phosphatase activity of protein phosphatase 2A in an ATP, Mg(2+)-requiring reaction (Cayla, X., Goris, J., Hermann, J., Hendrix, P., Ozon, R., and Merevede, W. (1990) Biochemistry 29, 658-667).
Sonish Azam et al.
Apoptosis : an international journal on programmed cell death, 12(7), 1243-1255 (2007-03-03)
PTPA, which possesses a peptidyl prolyl isomerase activity, was initially isolated as a protein that stimulates the weak phosphotyrosyl phosphatase activity of the Ser/Thr phosphatase PP2A. Here we show that transient overexpression of PTPA leads to cell death in a
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