SAB4200275
Anti-EIF2C2/Ago2 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~97 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
western blot: 2-4 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EIF2C2(27161)
General description
The eukaryotic translation initiation factor 2C, subunit 2 (EIF2C2) or protein argonaute-2 (Ago2) belongs to the argonaute protein subfamily. The argonaute proteins contains the piwi-argonaute-zwille (PAZ), the P-element induced wimpy testis (PIWI) domain and the MID domain. Ago2 is localized to the processing bodies (P bodies) and the nucleus. The AGO2 gene is mapped to human chromosome 8q24.3.
Immunogen
synthetic peptide corresponding to the N-terminal region of human EIF2C2/Ago2 conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse.
Application
Anti-EIF2C2/Ago2 (N-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
Anti-EIF2C2/Ago2 recognizes human EIF2C2/Ago2.
The protein argonaute-2 (Ago2) is considered as a catalytic unit of mammalian RNA interference (RNAi). It possesses a slicer endonuclease activity that cleaves the target RNA in RNAi. The mRNA cleavage is possible by ribonucleoprotein complexes. Ago2 mediates tissue-specific developmental processes. Ago2 regulates both, the transcriptional and posttranslational modifications. It is implicated in the tumorigenic progression of breast cancer cell lines.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Brian D Adams et al.
Endocrinology, 150(1), 14-23 (2008-09-13)
Argonaute (Ago) 2 is the catalytic engine of mammalian RNA interference, but little is known concerning the regulation of Ago2 by cell-signaling pathways. In this study we show that expression of Ago2, but not Ago1, Ago3, or Ago4, is elevated
Manal S Fawzy et al.
American journal of translational research, 12(5), 1994-2006 (2020-06-09)
MicroRNA machinery genes Argonaute 1 (AGO1) and 2 (AGO2) are associated with several hallmarks of cancer. They play a key role in transcriptomic silencing, regulation of the immune system, cell differentiation, and angiogenesis processes. The present pilot study aims to
Jidong Liu et al.
Science (New York, N.Y.), 305(5689), 1437-1441 (2004-07-31)
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute family proteins. Here, we show that the multiple Argonaute proteins present in mammals are
Lasse Peters et al.
Molecular cell, 26(5), 611-623 (2007-06-15)
Small regulatory RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi interacting RNAs (piRNAs) have been discovered in the past, and it is becoming more and more apparent that these small molecules have key regulatory functions. Small RNAs
Gunter Meister et al.
Molecular cell, 15(2), 185-197 (2004-07-21)
Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To
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