Anti-Actin, Muscle specific antibody, Mouse monoclonal

Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.

SDS-extracted protein fraction of human myocardium.

The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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