SAB4200300
Anti-DKK1 antibody, Mouse monoclonal
clone DKK1, purified from hybridoma cell culture
Synonym(s):
ANTI-SK, Anti-DICKKOPF homolog 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DKK1, monoclonal
form
buffered aqueous solution
mol wt
antigen apparent mol wt 29/36 kDa (doublet)
antigen predicted mol wt ~29 kDa
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
western blot: 1.0-2.0 μg/mL using human A549 serum free condition medium.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DKK1(22943)
General description
Monoclonal Anti-DKK1 (mouse IgG2a isotype) is derived from the hybridoma DKK-33 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. The Dickkopf (DKK) family comprises five conserved members DKK1-4 and a DKK3-related member Dickkopf-like protein 1 (DKKL1/ Soggy). DKK1-4 encodes for secreted glycoproteins. DKK1 protein contains an N-linked glycosyl group on its carboxy terminal region. The DKK1 gene is mapped to human chromosome 10q21.1.
Immunogen
peptide corresponding to a sequence at the N-terminus human DKK1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse DKK1.
Application
Anti-DKK1 antibody, Mouse monoclonal is suitable for immunoblotting.
Biochem/physiol Actions
Dickkopf1 (DKK1) is a secreted inhibitor of the canonical wnt (wingless-related integration site) signaling pathway that modulates this pathway during embryonic development, including head induction, skeletal development, and limb patterning. DKK1, -2, and -4 have been shown to inhibit wnt signaling by interacting with and antagonizing low-density lipoprotein receptor-related protein (LRP) 5 and LRP6. In addition to LRP5/6, DKK1 binds to kringle containing transmembrane protein (Kremen1 and Kremen2) receptors. The ternary DKK/LRP/Krm complex is rapidly endocytosed, resulting in the removal of the LRP5/6 receptors from the cell membrane and in prolonged inhibition of wnt signaling. DKK1 is also implicated in several diseases including osteoporosis, arthritis, and cancer and is a potential therapeutic target for the treatment of these diseases.
Monoclonal Anti-DKK1 recognizes human and mouse DKK1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to onemonth. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Mitchell E Menezes et al.
International journal of cancer, 130(7), 1477-1483 (2011-09-29)
Dickkopf1 (DKK1), a secreted inhibitor of the Wnt/β-catenin pathway, is a negative regulator of bone formation. DKK1 acts as a switch that transitions prostate cancer bone metastases from osteolytic to osteoblastic and also is an active indicator of poor outcome
Cristina-Maria Cruciat et al.
Cold Spring Harbor perspectives in biology, 5(3), a015081-a015081 (2012-10-23)
Signaling by the Wnt family of secreted glycoproteins plays important roles in embryonic development and adult homeostasis. Wnt signaling is modulated by a number of evolutionarily conserved inhibitors and activators. Wnt inhibitors belong to small protein families, including sFRP, Dkk
Ben Steventon et al.
Developmental biology, 365(1), 196-207 (2012-03-08)
Neural crest (NC) induction is a long process that continues through gastrula and neurula stages. In order to reveal additional stages of NC induction we performed a series of explants where different known inducing tissues were taken along with the
Yi-Ying Lin et al.
International journal of molecular sciences, 19(9) (2018-08-29)
Several efforts have been made on the development of bioscaffolds including the polydimethylsiloxane (PDMS) elastomer for supporting cell growth into stable sheets. However, PDMS has several disadvantages, such as intrinsic surface hydrophobicity and mechanical strength. Herein, we generated a novel
José M López et al.
Bone, 43(2), 405-409 (2008-06-10)
Craniotubular hyperostosis are a group of high bone mass disorders related to mutations in the LRP5 and SOST genes, although other causative genes remain to be identified. Little is known about the bone turnover and the response to T3 or
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