Skip to Content
Merck
CN

SAB4200395

Sigma-Aldrich

Anti-AMSH-LP / STAMBPL1 antibody, Mouse monoclonal

clone AMSH-LP-4, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-ALM alpha, Monoclonal Anti-AMSH-FP, Monoclonal Anti-AMSH-LP / STAMBPL1 antibody produced in mouse, Monoclonal Anti-STAM binding protein-like 1

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AMSH-LP-4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, rat, human

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using HEK-293T cells over-expressing AMSH-LP /STAMBPL1.

isotype

IgG1

UniProt accession no.

Q96FJ0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... STAMBPL1(57559)
mouse ... Stambpl1(76630)

General description

Monoclonal Anti-AMSH-LP/STAMBPL1 (mouse IgG1 isotype) is derived from the hybridoma AMSH-LP-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Associated molecule with the SH3 domain of STAM-like protease (AMSH-LP), also known as signal-transducing adaptor molecule binding protein-like 1 (STAMBPL1), is a close homolog of AMSH. AMSH and AMSH-LP belong to the Jab1/MPN domain metalloenzyme (JAMM) domain metalloprotease family of Zn2+-dependent deubiquitinating enzymes (DUBs). It comprises a nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain and a JAMM motif. The gene AMSH-LP is expressed in several human tissues. It is mapped to the human chromosome location 10q23.31.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human STAMBPL1, conjugated to KLH. The corresponding sequence is identical in mouse, rat monkey, bovine and canine STAMBPL1.

Application

Monoclonal Anti-AMSH-LP/STAMBPL1 antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Associated molecule with the SH3 domain of STAM-like protease (AMSH-LP) is involved in the deubiquitination of endosomal proteins and specifically cleaves Lys 63-linked polyubiquitin chains. It interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. AMSH-LP positively regulates tumor growth factor β (TGF-β) signaling through interaction with inhibitory small mothers against decapentaplegic (I-SMAD). It functions as an adaptor for gene transcription and regulation system.
Monoclonal Anti-AMSH-LP /STAMBPL1 recognizes human AMSH-LP /STAMBPL1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
111M4783

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A newly identified AMSH-family protein is specifically expressed in haploid stages of testicular germ cells
Kitajima K, et al.
Biochemical and biophysical research communications, 309(1), 135-142 (2003)
Emerging roles of deubiquitinases in cancer-associated pathways
Sacco JJ, et al.
IUBMB Life, 62(2), 140-157 (2010)
Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains
Sato Y, et al.
Nature, 455(7211), 358-362 (2008)
Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif
Kikuchi K, et al.
Biochemical and Biophysical Research Communications, 306(3), 637-643 (2003)
Barbara Kloeckener-Gruissem et al.
American journal of human genetics, 82(3), 772-779 (2008-02-29)
Unobstructed vision requires a particular refractive index of the lens, a measure based on the organization of the structural proteins within the differentiated lens cells. To ensure an intact lens structure, homeostasis within the lens cells is indispensable. Alterations of
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service