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Merck
CN

SAB4200413

Monoclonal Anti-LIN28 antibody produced in mouse

clone L28.5, tissue culture supernatant

Synonym(s):

Monoclonal Anti-CSDD1, Monoclonal Anti-LIN28A, Monoclonal Anti-ZCCHC1

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352202
Conjugate:
unconjugated
Clone:
L28.5, monoclonal
Application:
WB
Citations:
2

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

L28.5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, human

technique(s)

western blot: 1:8,000-1:16,000 using extracts of Ntera-2 cells.

isotype

IgM

UniProt accession no.

Q9H9Z2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LIN28A(79727)
mouse ... Lin28a(83557)

General description

LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended.
Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A.

Biochem/physiol Actions

The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.

Physical form

Solution containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
022M4763V
022M4763

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Florian Mayr et al.
International journal of molecular sciences, 14(8), 16532-16553 (2013-08-14)
Lin28 is an essential RNA-binding protein that is ubiquitously expressed in embryonic stem cells. Its physiological function has been linked to the regulation of differentiation, development, and oncogenesis as well as glucose metabolism. Lin28 mediates these pleiotropic functions by inhibiting
Linbo Wang et al.
PloS one, 8(6), e67373-e67373 (2013-07-11)
Resistance to radiation therapy is a major obstacle for the effective treatment of cancers. Lin28 has been shown to contribute to breast tumorigenesis; however, the relationship between Lin28 and radioresistance remains unknown. In this study, we investigated the association of

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