SAB4200416
Anti-Tensin 3 antibody, Mouse monoclonal
clone TN-17, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-TEM6, Monoclonal Anti-TENS1, Monoclonal Anti-TNS3, Monoclonal Anti-Tensin 3 antibody produced in mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TN-17, monoclonal
form
buffered aqueous solution
mol wt
antigen ~170 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2.5-5.0 μg/mL using PC3 cells.
western blot: 0.5-1.0 μg/mL using extracts of SW-620 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNS3(64759)
General description
Monoclonal Anti-Tensin 3 (mouse IgG1 isotype) is derived from the hybridoma TN-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human Tensin 3. Tensins are multidomain proteins that are characterized with homologous C1, protein phosphatases (PTPases) , C2, Src homology 2 (SH2) domains and pTyr-binding (PTB) domains.
Tensin 3 (TNS3) is encoded by the gene mapped to human chromosome 7p12.3. TNS3 transcript is expressed at high level in thyroid and at moderate level in placenta. In addition, the protein is also expressed at minimal levels in brain, heart, lung, liver, pancreas, kidney, skeletal muscle, white blood cells and prostate. TNS3 is a member of tensin protein family.
Immunogen
synthetic peptide corresponding to an internal sequence of human Tensin 3, conjugated to KLH. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Application
Monoclonal Anti-Tensin 3 antibody produced in mouse has also been used in immunofluorescence.
Monoclonal Anti-Tensin 3 antibody produced in mouse has been used in western blotting.
Biochem/physiol Actions
Tensin 3 (TNS3) plays a vital role in stimulation of epidermal growth factor (EGF) signaling pathway. The encoded protein supports fundamental role of thyroid hormone in normal thyroid tissues. Decreased expression of the gene leads to development of human renal cell carcinoma (RCC).
Tensin 3 facilitates cell migration and tumorigenesis in cell lines from advanced lung cancer, breast cancer and melanoma. In these cell lines, The protein is phosphorylated by Src in its SH2 domain, leading to tumorigenesis and metastasis, implicating tensin 3 as an oncoprotein regulated by Src.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Epidermal Growth Factor Modulates Tyrosine Phosphorylation of a Novel Tensin Family Member, Tensin311Supported in part by the Shriners Hospitals Research Award and NIH CA102537.
Cui Y
Molecular Cancer Research (2004)
Tensin3 is a novel thyroid-specific gene
Maeda I
Journal of Molecular Endocrinology, 36, R1-R8 (2008)
Musashi-1 Enhances Glioblastoma Cell Migration and Cytoskeletal Dynamics through Translational Inhibition of Tensin3
Chen HY
Scientific Reports, 7, 8710-8710 (2017)
Increased expression levels of tensin3 mRNA in thyroid functional adenomas as compared to non-functioning adenomas.
Maeda I
Experimental and Clinical Endocrinology & Diabetes, 117, 191-193 (2009)
CpG dinucleotide-specific hypermethylation of the TNS3 gene promoter in human renal cell carcinoma
Carter JA
Epigenetics, 8, 739-747 (2013)
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