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Merck
CN

SAB4200469

Sigma-Aldrich

Anti-POLR2A antibody produced in rabbit

Ig fraction of antiserum

Synonym(s):

Anti-POLR2, Anti-POLRA, Anti-RPBh1, Anti-RPO2, Anti-RPOL2, Anti-RpIILS, Anti-hRPB220, Anti-hsRPB1, Anti-polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RPB1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

Ig fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~250 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): 5-10 μL using whole extract of human HeLa cells
indirect immunofluorescence: 1:400-1:800 using human HeLa cells
western blot: 1:2000-1:4000 using nuclear extracts of HeLa cells

UniProt accession no.

P24928

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POLR2A(5430)
mouse ... Polr2a(20020)
rat ... Polr2a(363633)

General description

RNA polymerase II subunit A (POLR2A) gene with 29 exons is mapped on human chromosome 17p13.1. This gene codes for a catalytic subunit of RNA polymerase II. The encoded protein consists of a highly conserved 5′ flanking region.

Immunogen

peptide corresponding to the N-terminal region of human POLR2A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, dog and pig.

Biochem/physiol Actions

RNA polymerase II subunit A (POLR2A) catalyzes the synthesis of messenger RNA in eukaryotes. The 5′ flanking domain of RNA polymerase II subunit A (POLR2A) plays a vital role in transcriptional regulation. Mutation in the gene is associated with the pathogenesis of meningiomas.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
072M4766V

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The effect of aryl hydrocarbon receptor ligands on the expression of polymerase (DNA directed) kappa (Pol?), polymerase RNA II (DNA directed) polypeptide A (PolR2a), CYP1B1 and CYP1A1 genes in rat liver
Brauze D
Environmental Toxicology and Pharmacology, 34, 819-825 (2012)
The human gene encoding the largest subunit of RNA polymerase II
Mita K
Gene, 159, 285-286 (1995)
Recurrent somatic mutations in POLR2A define a distinct subset of meningiomas
Clark VE
Nature Genetics, 48, 1253-1259 (2016)
1p31, 7q21 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine resistance of human cervical carcinoma KB cells.
Wang J
Oncology Reports, 19, 1155-1164 (2008)
Farhana Taher Sumya et al.
Traffic (Copenhagen, Denmark), 24(2), 52-75 (2022-12-06)
Conserved Oligomeric Golgi (COG) complex controls Golgi trafficking and glycosylation, but the precise COG mechanism is unknown. The auxin-inducible acute degradation system was employed to investigate initial defects resulting from COG dysfunction. We found that acute COG inactivation caused a

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