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Merck
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SAB4200490

Sigma-Aldrich

Anti-DLC1 (C-terminal region) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Deleted in Liver Cancer 1, ARHGAP7, Anti-HP, Anti-STARD12, Anti-p122-RhoGAP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon.
immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells overexpressing human DLC1.
indirect immunofluorescence: 1-2 μg/mL using MDBK cells.
western blot: 1.5-3 μg/mL using lysates of HEK-293T cells overexpressing human DLC1.

UniProt accession no.

Q96QB1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DLC1(10395)

General description

Deleted in liver cancer 1 (DLC1), also known as ARHGAP7, is a candidate tumor suppressor gene, originally isolated from human hepatocellular carcinoma (HCC). DLC1 is mapped to chromosome 8p21.3-22 and is also expressed in normal human tissues. DLC1 protein comprises three domains namely steroidogenic acute regulatory related lipid transfer (START) domain, sterile a-motif (SAM) and Rho GTPase-activating protein (RhoGAP) domain.

Immunogen

synthetic peptide corresponding to a sequence near the C-terminal of human DLC1 isoform 1, conjugated to KLH.

Application

Anti-DLC1 (C-terminal region) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Anti-DLC1(C-terminal region) specifically recognizes human DLC1.
Deleted in liver cancer 1 (DLC1) interacts with focal adhesion protein, tensin 2. DLC1 gene locus is recurrently deleted in solid tumors as well as in hepatocellular carcinoma (HCC). The Rho GTPase-activating protein (RhoGAP) region is responsible for the growth inhibitory effect in HCC. It has been shown that the N-terminal region of DLC1 localizes to focal adhesions by binding to SH2 domains on tensins. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The cell motility and morphology maintenance functionality of DLC1 is primarily mediated by steroidogenic acute regulatory protein (STAR)- GTPase-activating proteins (GAP1) region.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
072M4763V
072M4763

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Katsuhisa Kawai et al.
Genes to cells : devoted to molecular & cellular mechanisms, 14(2), 227-241 (2009-01-28)
There is a class of GTPase activating proteins for the Rho family GTPases (RhoGAPs) that contain the steroidogenic acute regulatory protein (STAR)-related lipid transfer (START) domain. In mammals three genes encode such proteins and they are designated START-GAP1-3 or deleted
Judy Wai Ping Yam et al.
Cancer research, 66(17), 8367-8372 (2006-09-05)
Deleted in liver cancer 1 (DLC1) is a recently identified tumor suppressor gene frequently underexpressed in hepatocellular carcinoma (HCC). DLC1 encodes a Rho GTPase-activating protein domain that exhibits growth-suppressive activity in HCC cell lines. Our recent finding has revealed that
Yi-Chun Liao et al.
Cancer research, 68(19), 7718-7722 (2008-10-03)
Deleted in liver cancer-1 (DLC-1) is a RhoGTPase-activating protein (RhoGAP) domain containing tumor suppressor that is often down-regulated in various cancer types. Previously, we have shown that DLC-1 is recruited to focal adhesions by binding to the Src homology 2
Wen Xue et al.
Genes & development, 22(11), 1439-1444 (2008-06-04)
Deletions on chromosome 8p are common in human tumors, suggesting that one or more tumor suppressor genes reside in this region. Deleted in Liver Cancer 1 (DLC1) encodes a Rho-GTPase activating protein and is a candidate 8p tumor suppressor. We

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