Monoclonal Anti-NF1 antibody produced in mouse

Monoclonal Anti-NF1 (mouse IgM isotype) is derived from the hybridoma NF1-333 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neurofibromin 1 (NF1) gene is located at chromosome 17q11.2. It is predominantly expressed in neurons, Schwann cells, oligodendrocytes, astrocytes and leukocytes. NF1 has two potentially functional domains; Ras GTPase activating protein (RasGAP)and phosphatidylinositol-transfer protein.

synthetic peptide corresponding to a sequence at the N-terminus of human NF1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Monoclonal Anti-NF1 antibody produced in mouse has been used in western blotting. It may be suitable for use in flow cytometry.

NF1 acts as a negative regulator of the p21-Ras/mitogen-activated protein kinase (MAPK) pathway as well as a tumor suppressor gene. Mutations in the neurofibromin 1 (NF1) gene is implicated in Neurofibromatosis type 1 (NF1) gene also known as von Recklinghausen peripheral or classical neurofibromatosis, is one of the most common autosomal dominant diseases. Consistent with a central role for neurofibromin in cellular function, recent cancer genome sequencing studies have found that somatic NF1 gene mutations occur not only in association with NF1, but also in a number of other common cancers. A variety of benign and malignant tumours are associated with NF1 and all involve tumorigenesis of neural crest-derived cells.

Culture supernatant solution containing 15 mM sodium azide.

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