SAB4200537
Anti-Claudin-5 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-AWAL, Anti-BEC1, Anti-CLDN5, Anti-CPETRL1, Anti-TMVCF
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~23 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human heart
western blot: 1-2 μg/mL using extracts of OVCAR-3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CLDN5(7122)
General description
Claudin-5 is a member of claudin family. It is also known as CLDN5, beclin 1, TMVCF and CPETRL1 and is highly expressed in endothelial cells (ECs), including the blood-brain barrier.
Claudin-5 is encoded by the gene mapped to human chromosome 22q11.21.
Immunogen
synthetic peptide corresponding to an internal sequence of human claudin-5, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in mouse and rat claudin-5.
Application
Anti-Claudin-5 antibody produced in rabbit has been used in immunofluorescence staining.
Anti-Claudin-5 antibody produced in rabbit may be used in several immunochemical techniques including immunoblotting (~23 kDa) and immunohistochemistry.
Biochem/physiol Actions
Anti-Claudin-5specifically recognizes human Claudin-5.
Claudin-5 gene is a downstream target of ETS-related gene (ERG), a transcription factor that regulates endothelial cell-restricted genes and is markedly repressed in response to inflammatory stimuli. Knockout of claudin-5 in mice is lethal, resulting in disruption of in the blood-brain barrier. Claudin-5 has been shown to be overexpressed in various tumors including lung adenocarcinomas but undetectable in squamous cells cell carcinomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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ETS-related gene (ERG) controls endothelial cell permeability via transcriptional regulation of the claudin 5 (CLDN5) gene
Yuan L, et al.
Test, 287(9), 6582-6591 (2012)
Marta Justyna Kozieł et al.
Cancers, 12(3) (2020-03-22)
Claudins are major integral proteins of tight junctions (TJs), the apical cell-cell adhesions that enable maintaining polarity of epithelial cells, their differentiation, and cell signaling. A number of studies have indicated that claudins might play a crucial role in both
Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas
Paschoud S, et al.
Modern Pathology, 20(9), 947-947 (2007)
Janyerkye Tulyeu et al.
Microorganisms, 7(10) (2019-10-19)
Increased intestinal permeability is thought to underlie the pathogenesis of food allergy. We explore the mechanism responsible for changes in the morphology and function of the intestinal barrier using a rat model of food allergy, focusing on the contribution of
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