SAB4200545
Anti-Parvalbumin antibody, Mouse monoclonal
clone PARV-19, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-ALPHA-PARVALBUMIN, Monoclonal Anti-PALB1, Monoclonal Anti-PV, Monoclonal Anti-PVALB (includes EG:19293), Monoclonal Anti-Parv, Monoclonal Anti-Pva
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.44
UNSPSC Code:
12352203
Product Name
Anti-Parvalbumin antibody, Mouse monoclonal, clone PARV-19, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PARV-19, monoclonal
form
buffered aqueous solution
mol wt
antigen 12 kDa
species reactivity
rat, human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum.
indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells.
western blot: 1-2 μg/mL using mouse muscle extracts.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PVALB(5816)
rat ... PVALB(25269)
Application
Anti-Parvalbumin antibody, Mouse monoclonal has been used in:
- immunohistochemistry
- immunofluorescence staining
- immunolabeling
- to label brain slices for perineuronal net staining
- enzyme-linked immunosorbent assay (ELISA)
- immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Ca2+ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca2+ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson.
Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca2+-binding molecules. It carries three helix-loop-helix EF-hand Ca2+ binding motifs, with the two C-terminal motifs.
Immunogen
purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Syed Hasan Arif
BioEssays : news and reviews in molecular, cellular and developmental biology, 31(4), 410-421 (2009-03-11)
Parvalbumins (PVs) are acidic, intracellular Ca(2+)-binding proteins of low molecular weight. They are associated with several Ca(2+)-mediated cellular activities and physiological processes. It has been suggested that PV might function as a "Ca2+ shuttle" transporting Ca2+ from troponin-C (TnC) to
Rachel Schroeder et al.
Antioxidants & redox signaling, 35(7), 511-530 (2021-01-28)
Aims: Impaired embryonic cortical interneuron development from prenatal stress is linked to adult neuropsychiatric impairment, stemming in part from excessive generation of reactive oxygen species in the developing embryo. Unfortunately, there are no preventive medicines that mitigate the risk of
Jada L-B Davis et al.
The Yale journal of biology and medicine, 95(1), 87-104 (2022-04-05)
Prenatal stress is a neuropsychiatric risk factor, and effects may be mediated by prenatal oxidative stress. Cell types in the brain sensitive to oxidative stress-cortical microglia and cortical and hippocampal interneurons-may be altered by oxidative stress generated during prenatal stress
Wang Wang et al.
General physiology and biophysics, 28 Spec No Focus, F3-F6 (2010-01-23)
Diastolic heart failure (DHF) is a clinical syndrome characterized by depressed myocardial relaxation performance and poor ventricular refilling. Defective intracellular calcium (Ca(2+)) handling underlies one of the fundamental mechanisms of DHF. Manipulating the content and function of Ca(2+) handling proteins
O Caillard et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(24), 13372-13377 (2000-11-09)
GABAergic (GABA = gamma-aminobutyric acid) neurons from different brain regions contain high levels of parvalbumin, both in their soma and in their neurites. Parvalbumin is a slow Ca(2+) buffer that may affect the amplitude and time course of intracellular Ca(2+)
Related Content
Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service