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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Species reactivity:
human
Technique(s):
immunoblotting: 4-8 μg/mL using whole extracts of human U-2-OS cells.
Application:
—
Citations:
2
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoblotting: 4-8 μg/mL using whole extracts of human U-2-OS cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... WHAMM(123720)
General description
WASP homologue associated with actin, membranes, and microtubules (WHAMM) is a nucleation promoting factor. It is expressed in the cis-Golgi and tubulo-vesicular membrane transport intermediates. WHAMM is composed of an N-terminal region, a coiled-coil region and a WCA region. It is located on the human chromosome at 15q25.2.
Immunogen
peptide corresponding to an internal region of human WHAMM ,conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse and by 4 in rat WHAMM.
Application
Anti-WHAMM antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Anti- WHAMM recognizes human WHAMM.
WASP homologue associated with actin, membranes, and microtubules (WHAMM) is involved in actin-related protein 2 & 3 (Arp2/3)-mediated actin polymerization, mediates Golgi membrane association and microtubule dynamics. It is also involved in Golgi structure maintenance and participates in vesicle transport between the endoplasmic reticulum and the Golgi complex.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
Regulatory Information
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Related Content
Seminars in cell & developmental biology
Seminars in Cell & Developmental Biology, 24(4), 258-266 (2013)
WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport.
Kenneth G Campellone et al.
Cell, 134(1), 148-161 (2008-07-11)
The Arp2/3 complex is an actin nucleator that plays a critical role in many cellular processes. Its activities are regulated by nucleation-promoting factors (NPFs) that function primarily during plasma membrane dynamics. Here we identify a mammalian NPF called WHAMM (WASP
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4200589-200UL | 04061837725692 |